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morphogen concentration  (Miltenyi Biotec)


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    Miltenyi Biotec morphogen concentration
    a ) Photographs of the sample holding chamber with four separated sub-chambers each containing four microwells, for growing and imaging organoids with sixteen different organoids arranged one per microwell n = 16 organoids. b ) Schematic overview of tiled image acquisition and example images showing two timepoints, before and after tiled acquisition and image fusion. Scale Bar is 100 micrometers. Time is in hours. n = 16 organoids. c ) Cross section images (day 7) from 16 simultaneously imaged organoids, generated with cells lines labeled with nuclear membrane (lamin, RFP, magenta), plasma membrane (CAAX, RFP, magenta), actin (GFP, green), tubulin (RFP, magenta), and nuclei (histone, GFP, green) and unlabeled WTC-11. d ) Images showing cross section (z-plane) and orthogonal views (y-plane, x-plane) of one organoid from a timecourse lightsheet imaging experiment shown in c. Scale Bar is 100 micrometers. Time is in hours. e ) Maximum intensity projections from a 2-week imaging acquisition of a mosaic organoid (histone, GFP, green; CAAX, RFP, magenta), unlabeled WTC-11). Scale Bar is 500 micrometers. Time is in hours. n = 16 organoids. f ) Images from a 3-week continuous imaging experiment using a NKX2-1:GFP reporter line. The organoids were given SHH <t>morphogen</t> treatment to induce ventral telencephalic patterning of the organoids. Images are false-colored with the green-fire-blue LUT. Scale Bar is 100 micrometers. Time is in hours. n = 4 organoids.
    Morphogen Concentration, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/morphogen concentration/product/Miltenyi Biotec
    Average 94 stars, based on 26 article reviews
    morphogen concentration - by Bioz Stars, 2026-02
    94/100 stars

    Images

    1) Product Images from "Morphodynamics of human early brain organoid development"

    Article Title: Morphodynamics of human early brain organoid development

    Journal: Nature

    doi: 10.1038/s41586-025-09151-3

    a ) Photographs of the sample holding chamber with four separated sub-chambers each containing four microwells, for growing and imaging organoids with sixteen different organoids arranged one per microwell n = 16 organoids. b ) Schematic overview of tiled image acquisition and example images showing two timepoints, before and after tiled acquisition and image fusion. Scale Bar is 100 micrometers. Time is in hours. n = 16 organoids. c ) Cross section images (day 7) from 16 simultaneously imaged organoids, generated with cells lines labeled with nuclear membrane (lamin, RFP, magenta), plasma membrane (CAAX, RFP, magenta), actin (GFP, green), tubulin (RFP, magenta), and nuclei (histone, GFP, green) and unlabeled WTC-11. d ) Images showing cross section (z-plane) and orthogonal views (y-plane, x-plane) of one organoid from a timecourse lightsheet imaging experiment shown in c. Scale Bar is 100 micrometers. Time is in hours. e ) Maximum intensity projections from a 2-week imaging acquisition of a mosaic organoid (histone, GFP, green; CAAX, RFP, magenta), unlabeled WTC-11). Scale Bar is 500 micrometers. Time is in hours. n = 16 organoids. f ) Images from a 3-week continuous imaging experiment using a NKX2-1:GFP reporter line. The organoids were given SHH morphogen treatment to induce ventral telencephalic patterning of the organoids. Images are false-colored with the green-fire-blue LUT. Scale Bar is 100 micrometers. Time is in hours. n = 4 organoids.
    Figure Legend Snippet: a ) Photographs of the sample holding chamber with four separated sub-chambers each containing four microwells, for growing and imaging organoids with sixteen different organoids arranged one per microwell n = 16 organoids. b ) Schematic overview of tiled image acquisition and example images showing two timepoints, before and after tiled acquisition and image fusion. Scale Bar is 100 micrometers. Time is in hours. n = 16 organoids. c ) Cross section images (day 7) from 16 simultaneously imaged organoids, generated with cells lines labeled with nuclear membrane (lamin, RFP, magenta), plasma membrane (CAAX, RFP, magenta), actin (GFP, green), tubulin (RFP, magenta), and nuclei (histone, GFP, green) and unlabeled WTC-11. d ) Images showing cross section (z-plane) and orthogonal views (y-plane, x-plane) of one organoid from a timecourse lightsheet imaging experiment shown in c. Scale Bar is 100 micrometers. Time is in hours. e ) Maximum intensity projections from a 2-week imaging acquisition of a mosaic organoid (histone, GFP, green; CAAX, RFP, magenta), unlabeled WTC-11). Scale Bar is 500 micrometers. Time is in hours. n = 16 organoids. f ) Images from a 3-week continuous imaging experiment using a NKX2-1:GFP reporter line. The organoids were given SHH morphogen treatment to induce ventral telencephalic patterning of the organoids. Images are false-colored with the green-fire-blue LUT. Scale Bar is 100 micrometers. Time is in hours. n = 4 organoids.

    Techniques Used: Imaging, Generated, Labeling, Membrane, Clinical Proteomics



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    morphogen concentration  (Miltenyi Biotec)
    94
    Miltenyi Biotec morphogen concentration
    a ) Photographs of the sample holding chamber with four separated sub-chambers each containing four microwells, for growing and imaging organoids with sixteen different organoids arranged one per microwell n = 16 organoids. b ) Schematic overview of tiled image acquisition and example images showing two timepoints, before and after tiled acquisition and image fusion. Scale Bar is 100 micrometers. Time is in hours. n = 16 organoids. c ) Cross section images (day 7) from 16 simultaneously imaged organoids, generated with cells lines labeled with nuclear membrane (lamin, RFP, magenta), plasma membrane (CAAX, RFP, magenta), actin (GFP, green), tubulin (RFP, magenta), and nuclei (histone, GFP, green) and unlabeled WTC-11. d ) Images showing cross section (z-plane) and orthogonal views (y-plane, x-plane) of one organoid from a timecourse lightsheet imaging experiment shown in c. Scale Bar is 100 micrometers. Time is in hours. e ) Maximum intensity projections from a 2-week imaging acquisition of a mosaic organoid (histone, GFP, green; CAAX, RFP, magenta), unlabeled WTC-11). Scale Bar is 500 micrometers. Time is in hours. n = 16 organoids. f ) Images from a 3-week continuous imaging experiment using a NKX2-1:GFP reporter line. The organoids were given SHH <t>morphogen</t> treatment to induce ventral telencephalic patterning of the organoids. Images are false-colored with the green-fire-blue LUT. Scale Bar is 100 micrometers. Time is in hours. n = 4 organoids.
    Morphogen Concentration, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/morphogen concentration/product/Miltenyi Biotec
    Average 94 stars, based on 1 article reviews
    morphogen concentration - by Bioz Stars, 2026-02
    94/100 stars
    		  Buy from Supplier
    morphogen concentration gradients  (Basler)
    90
    Basler morphogen concentration gradients
    a ) Photographs of the sample holding chamber with four separated sub-chambers each containing four microwells, for growing and imaging organoids with sixteen different organoids arranged one per microwell n = 16 organoids. b ) Schematic overview of tiled image acquisition and example images showing two timepoints, before and after tiled acquisition and image fusion. Scale Bar is 100 micrometers. Time is in hours. n = 16 organoids. c ) Cross section images (day 7) from 16 simultaneously imaged organoids, generated with cells lines labeled with nuclear membrane (lamin, RFP, magenta), plasma membrane (CAAX, RFP, magenta), actin (GFP, green), tubulin (RFP, magenta), and nuclei (histone, GFP, green) and unlabeled WTC-11. d ) Images showing cross section (z-plane) and orthogonal views (y-plane, x-plane) of one organoid from a timecourse lightsheet imaging experiment shown in c. Scale Bar is 100 micrometers. Time is in hours. e ) Maximum intensity projections from a 2-week imaging acquisition of a mosaic organoid (histone, GFP, green; CAAX, RFP, magenta), unlabeled WTC-11). Scale Bar is 500 micrometers. Time is in hours. n = 16 organoids. f ) Images from a 3-week continuous imaging experiment using a NKX2-1:GFP reporter line. The organoids were given SHH <t>morphogen</t> treatment to induce ventral telencephalic patterning of the organoids. Images are false-colored with the green-fire-blue LUT. Scale Bar is 100 micrometers. Time is in hours. n = 4 organoids.
    Morphogen Concentration Gradients, supplied by Basler, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/morphogen concentration gradients/product/Basler
    Average 90 stars, based on 1 article reviews
    morphogen concentration gradients - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    a ) Photographs of the sample holding chamber with four separated sub-chambers each containing four microwells, for growing and imaging organoids with sixteen different organoids arranged one per microwell n = 16 organoids. b ) Schematic overview of tiled image acquisition and example images showing two timepoints, before and after tiled acquisition and image fusion. Scale Bar is 100 micrometers. Time is in hours. n = 16 organoids. c ) Cross section images (day 7) from 16 simultaneously imaged organoids, generated with cells lines labeled with nuclear membrane (lamin, RFP, magenta), plasma membrane (CAAX, RFP, magenta), actin (GFP, green), tubulin (RFP, magenta), and nuclei (histone, GFP, green) and unlabeled WTC-11. d ) Images showing cross section (z-plane) and orthogonal views (y-plane, x-plane) of one organoid from a timecourse lightsheet imaging experiment shown in c. Scale Bar is 100 micrometers. Time is in hours. e ) Maximum intensity projections from a 2-week imaging acquisition of a mosaic organoid (histone, GFP, green; CAAX, RFP, magenta), unlabeled WTC-11). Scale Bar is 500 micrometers. Time is in hours. n = 16 organoids. f ) Images from a 3-week continuous imaging experiment using a NKX2-1:GFP reporter line. The organoids were given SHH morphogen treatment to induce ventral telencephalic patterning of the organoids. Images are false-colored with the green-fire-blue LUT. Scale Bar is 100 micrometers. Time is in hours. n = 4 organoids.

    Journal: Nature

    Article Title: Morphodynamics of human early brain organoid development

    doi: 10.1038/s41586-025-09151-3

    Figure Lengend Snippet: a ) Photographs of the sample holding chamber with four separated sub-chambers each containing four microwells, for growing and imaging organoids with sixteen different organoids arranged one per microwell n = 16 organoids. b ) Schematic overview of tiled image acquisition and example images showing two timepoints, before and after tiled acquisition and image fusion. Scale Bar is 100 micrometers. Time is in hours. n = 16 organoids. c ) Cross section images (day 7) from 16 simultaneously imaged organoids, generated with cells lines labeled with nuclear membrane (lamin, RFP, magenta), plasma membrane (CAAX, RFP, magenta), actin (GFP, green), tubulin (RFP, magenta), and nuclei (histone, GFP, green) and unlabeled WTC-11. d ) Images showing cross section (z-plane) and orthogonal views (y-plane, x-plane) of one organoid from a timecourse lightsheet imaging experiment shown in c. Scale Bar is 100 micrometers. Time is in hours. e ) Maximum intensity projections from a 2-week imaging acquisition of a mosaic organoid (histone, GFP, green; CAAX, RFP, magenta), unlabeled WTC-11). Scale Bar is 500 micrometers. Time is in hours. n = 16 organoids. f ) Images from a 3-week continuous imaging experiment using a NKX2-1:GFP reporter line. The organoids were given SHH morphogen treatment to induce ventral telencephalic patterning of the organoids. Images are false-colored with the green-fire-blue LUT. Scale Bar is 100 micrometers. Time is in hours. n = 4 organoids.

    Article Snippet: The organoids were treated with the average morphogen concentration of SHH (140 ng/ml, Miltenyi, 130-095-727) from day 3–14 together with purmorphamine (0.21 μM, Miltenyi, 130-104-465).

    Techniques: Imaging, Generated, Labeling, Membrane, Clinical Proteomics